D-BALM new websites now includes 26 new method descriptions of advanced and alternative methods at all stages of development and acceptance have been published. Comprehensive descriptions of novel approach methods with summaries and/or protocols compliant with regulatory guidelines, undergoing scientific validation or developed and evaluated in EU integrated research projects, are provided. The main topic areas include: Stem cell cultures, Effects on Endocrine Systems, Eye Irritation, Hepatotoxicity, Mutagenicity and Systemic Toxicity comprising the “Tools and Methods Catalogue of the SEURAT-1 Research Initiative”.